WGS of VLP isolated from Leishmania infantum - SRA

SRX21613503: WGS of VLP isolated from Leishmania infantum
1 ILLUMINA (NextSeq 500) run: 3.4M spots, 1G bases, 338.6Mb downloads

Design: Virus-like particles are isolated from samples by centrifuging culture supernatant and filtering through a 0.2uM filter, with the filtrate concentrated in SM buffer using Amicon-15 centrifugal filter and incubated with DNase I and RNase. Nucleic acid was extracted using AllPrep DNA/RNA Mini Kit. RNA was reverse transcribed into cDNA using a SuperScript III First-Strand Synthesis System Kit.

Submitted by: University of Pennsylvania

Study: Virome of the kitome: detection of small, circular, satellite-like viruses in laboratory reagents

PRJNA1012505 • SRP458241 • All experiments • All runs

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We isolated viral particles from Leishmania samples and identified viral contigs through metagenomic sequencing, with subsequent detection of these viral contigs in negative controls (culture media and H2O) suggesting that the identified viruses have a laboratory origin.

Sample:

SAMN37261148 • SRS18786038 • All experiments • All runs

Organism: uncultured virus

Library:

Name: leish_i_rna

Instrument: NextSeq 500

Strategy: WGS

Source: METAGENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 3.4M spots, 1G bases, 338.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR25893141	3,432,451	1G	338.6Mb	2023-09-03

ID:: 29243568

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